Document 0417
 DOCN  M9480417
 TI    Lymphocytes and promonocytes attach to the synthetic [Tyr5,12, Lys7]-
       polyphemusin II peptide.
 DT    9410
 AU    Weeks BS; Nomizu M; Otaka A; Weston CA; Okusu A; Tamamura H; Matsumoto
       A; Yamamoto N; Fujii N; Department of Biology, Hamilton College,
       Clinton, New York 13323.
 SO    Biochem Biophys Res Commun. 1994 Jul 15;202(1):470-5. Unique Identifier
       : AIDSLINE MED/94311923
 AB    The [Tyr5,12,Lys7]-polyphemusin II peptide (T22) has been shown to
       inhibit HIV-1 replication in lymphocytes. The mechanism of T22
       inhibition of HIV-1 replication is not known but may involve T22
       competition with HIV-1 for attachment sites on the plasma membrane of
       targeted cells. Here we find that three human immunocyte cell lines (H9,
       Jurkat, and U-937) attach to T22. The phorbol ester,
       12-O-tetradecanoylphorbol 13-acetate (TPA), has been shown to activate
       intracellular protein kinase C and to stimulate lymphocyte attachment to
       various substrates through specific cell surface receptors. Here we find
       that TPA treatment enhances attachment of the immunocytes to T22 by
       three- to four-fold. These data demonstrate that T22 binds to immunocyte
       cell surfaces and support the hypothesis that T22 may inhibit HIV-1
       replication by competing with the virus for a common cell surface
       receptor(s).
 DE    Amino Acid Sequence  Cell Adhesion/DRUG EFFECTS  Cell Line  Human
       HIV-1/DRUG EFFECTS/PHYSIOLOGY  Isoquinolines/PHARMACOLOGY  Kinetics
       Lymphocytes/*PHYSIOLOGY  Molecular Sequence Data  Monocytes/*PHYSIOLOGY
       Peptides/*METABOLISM/PHARMACOLOGY  Phorbol Esters/PHARMACOLOGY
       Piperazines/PHARMACOLOGY  Tetradecanoylphorbol Acetate/PHARMACOLOGY
       Tumor Cells, Cultured  Virus Replication/DRUG EFFECTS  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).